کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5908034 1160971 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Intron length and accelerated 3′ gene evolution
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Intron length and accelerated 3′ gene evolution
چکیده انگلیسی

Genetic evolution depends in part upon a balance between negative selection and environmentally driven mutation. To explore whether this balance is affected by gene structure, we have used phylogenetic data mining to compare gene compositions across a range of species. Here we show that genomes of higher species exhibit a greater frequency of 5′ CpG islands and of CpG→TpG/CpA transitions. This latter mutational pattern exhibits a 5′-to-3′ trend in higher species, consistent with a length-dependent effect on methylation-dependent CpG suppression. Associated strand asymmetry (TpG > CpA) declines with gene length, implying attenuation of transcription-coupled repair 3′ to introns. A sharp 3′ rise in coding region single-nucleotide polymorphism frequency further supports a mechanistic role for intron length in promoting genetic variation by reducing repair and/or weakening negative selection. Consistent with this, the Ka/Ks ratio of 3′ exons exceeds that of centrally located exons in intron-containing, but not in intronless, genes (p < 0.0003). We conclude that the efficiency of transcription-coupled repair decreases with gene length, suggesting in turn that 3′ gene evolution is accelerated both by introns and by gene methylation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Genomics - Volume 88, Issue 6, December 2006, Pages 682-689
نویسندگان
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