کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5908038 | 1160971 | 2006 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Construction of a California condor BAC library and first-generation chicken-condor comparative physical map as an endangered species conservation genomics resource
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Construction of a California condor BAC library and first-generation chicken-condor comparative physical map as an endangered species conservation genomics resource Construction of a California condor BAC library and first-generation chicken-condor comparative physical map as an endangered species conservation genomics resource](/preview/png/5908038.png)
چکیده انگلیسی
To support genomic analysis of the endangered California condor (Gymnogyps californianus), a BAC library (CHORI-262) was generated using DNA from the blood of a female. The library consists of 89,665 recombinant BAC clones providing â¼Â 14-fold coverage of the presumed â¼Â 1.48-Gb genome. Taking advantage of recent progress in chicken genomics, we developed a first-generation comparative chicken-condor physical map using an overgo hybridization approach. The overgos were derived from chicken (164 probes) and New World vulture (8 probes) sequences. Screening a 2.8à subset of the total library resulted in 236 BAC-gene assignments with 2.5 positive BAC clones per successful probe. A preliminary comparative chicken-condor BAC-based map included 93 genes. Comparison of selected condor BAC sequences with orthologous chicken sequences suggested a high degree of conserved synteny between the two avian genomes. This work will aid in identification and characterization of candidate loci for the chondrodystrophy mutation to advance genetic management of this disease.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Genomics - Volume 88, Issue 6, December 2006, Pages 711-718
Journal: Genomics - Volume 88, Issue 6, December 2006, Pages 711-718
نویسندگان
Michael N. Romanov, Maxim Koriabine, Mikhail Nefedov, Pieter J. de Jong, Oliver A. Ryder,