کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5942611 1574714 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Lineage tracing of cells involved in atherosclerosis
ترجمه فارسی عنوان
ردیابی خطی سلولهای دخیل در آترواسکلروز
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
چکیده انگلیسی


- Cells within atherosclerotic lesions display a high degree of plasticity.
- Smooth muscle cells within atherosclerotic lesions can switch to a macrophage-like phenotype.
- Myeloid-derived cells within atherosclerotic lesions can express smooth muscle markers.

Background and aimsDespite the clinical importance of atherosclerosis, the origin of cells within atherosclerotic plaques is not fully understood. Due to the lack of a definitive lineage-tracing strategy, previous studies have provided controversial results about the origin of cells expressing smooth muscle and macrophage markers in atherosclerosis. We here aim to identify the origin of vascular smooth muscle (SM) cells and macrophages within atherosclerosis lesions.MethodsWe combined a genetic fate mapping approach with single cell expression analysis in a murine model of atherosclerosis.ResultsWe found that 16% of CD68-positive plaque macrophage-like cells were derived from mature SM cells and not from myeloid sources, whereas 31% of αSMA-positive smooth muscle-like cells in plaques were not SM-derived. Further analysis at the single cell level showed that SM-derived CD68+ cells expressed higher levels of inflammatory markers such as cyclooxygenase 2 (Ptgs2, p = 0.02), and vascular cell adhesion molecule (Vcam1, p = 0.05), as well as increased mRNA levels of genes related to matrix synthesis such as Col1a2 (p = 0.01) and Fn1 (p = 0.04), than non SM-derived CD68+ cells.ConclusionsThese results demonstrate that smooth muscle cells within atherosclerotic lesions can switch to a macrophage-like phenotype characterized by higher expression of inflammatory and synthetic markers genes that may further contribute to plaque progression.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Atherosclerosis - Volume 251, August 2016, Pages 445-453
نویسندگان
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