کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
595193 1454003 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Interaction between the natural lipopeptide [Glu1, Asp5] surfactin-C15 and hemoglobin: A spectroscopic and electrochemical investigation
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Interaction between the natural lipopeptide [Glu1, Asp5] surfactin-C15 and hemoglobin: A spectroscopic and electrochemical investigation
چکیده انگلیسی

Interactions between hemoglobin (Hb) and the natural lipopeptide [Glu1, Asp5] surfactin-C15 (surfactin) have been investigated by fluorescence spectroscopy and cyclic voltammetry. Surfactin can facilitate the conversion of aquometHb to hemichrome in 0.01 M phosphate buffer solution (PBS, pH 7.4) due to the interaction between Hb and surfactin. The electrochemistry results reveal that the changes between Fe (II) and Fe (III) of heme produce a reversible process in surfactin solution as indicated by cyclic voltammograms. In other words, the electron transfer which takes place is due to the interaction between Hb and surfactin. The binding constants of surfactin on hemoglobin were calculated by the Scatchard method and the characteristic signs of the thermodynamic parameters at two classes of binding sites for surfactin on Hb were obtained. The thermodynamic function of the binding process suggests that the hydrophobic interaction is the predominant intermolecular force between Hb and surfactin.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces A: Physicochemical and Engineering Aspects - Volume 369, Issues 1–3, 20 October 2010, Pages 154–159
نویسندگان
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