کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
595811 1454027 2009 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Preparation and characterization of porous biodegradable microspheres used for controlled protein delivery
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Preparation and characterization of porous biodegradable microspheres used for controlled protein delivery
چکیده انگلیسی

In this study, a new approach for protein delivery is introduced, involving biodegradable porous poly(d,l-lactic-co-glycolic acid) (PLGA) microspheres blocked with calcium alginate hydrogel. Porous microspheres were prepared by solvent evaporation method using sodium oleate as an extractable porogen. The resultant microspheres show porous surface and three-dimensional network structure with interconnecting pores. Human serum albumin (HSA) used as model protein, was adsorbed into micropores of porous microspheres by a solution immersing method. Thus, the harsh preparation conditions, such as the aqueous/organic interface produced by a water-in-oil microemulsion and the acute shearing strength brought by a high-speed homogenizer or a sonicater, inducing protein denaturation and aggregation could be avoided. Then, calcium alginate hydrogel was fabricated to block micropores of protein-loaded porous microspheres. A sustained protein release in vitro could be achieved in this way. Furthermore, it is noteworthy that the protein maintained its structural integrity after this preparation. The porous polymeric microspheres may have great potential application for water-soluble drugs, such as protein, vaccine and gene delivery.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces A: Physicochemical and Engineering Aspects - Volume 345, Issues 1–3, 5 August 2009, Pages 173–181
نویسندگان
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