Impact of osmotic pressure and gelling in the generation of highly stable single core water-in-oil-in-water (W/O/W) nano multiple emulsions of aspirin assisted by two-stage ultrasonic cavitational emulsification

► Generation of stable aspirin-loaded W/O/W nano multiple emulsions. ► Two-stage ultrasonic cavitational emulsification using Span 80 and Cremophore EL. ► Influences of glucose and gelatin on emulsion droplet size are investigated. ► 0.5% (w/w) inner glucose and 1% (w/w) gelatin in the inner phase lead to better stability. ► Gelling and osmotic pressure responsible for excellent stability of optimized formulation.