کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6018073 1580184 2013 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A non-cholinergic neuronal loss in the pedunculopontine nucleus of toxin-evoked Parkinsonian rats
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی عصب شناسی
پیش نمایش صفحه اول مقاله
A non-cholinergic neuronal loss in the pedunculopontine nucleus of toxin-evoked Parkinsonian rats
چکیده انگلیسی


- We reveal the total number of neurons in unilateral rat pedunculopontine nucleus.
- We examine changes in this number between 6-OHDA Parkinsonian rats and controls.
- We reveal that cholinergic neurons are not lost in this animal model of disease.
- Non-cholinergic neurons were lost in this nucleus in Parkinsonian rats.
- Other models may better reflect the cholinergic cell loss affecting human patients.

The pedunculopontine nucleus (PPN) controls various physiological functions, whilst being deemed a suitable target for low-frequency stimulation therapy for alleviating aspects of Parkinson's disease (PD). Previous studies showed that the PPN contains mainly cholinergic, γ-aminobutyric acid (GABA)ergic and glutamatergic neurons. Here we report on the total number of PPN neurons in laboratory rats, a species frequently used as an experimental model for simulating aspects of human PD. Moreover, the study reports that the number of PPN neurons decreases under toxic conditions that mimic in animals the core pathology seen in human PD. Immunohistochemical detection methods combined with unbiased stereology served to estimate that the PPN of healthy rats unilaterally contains ~ 19,028 NeuN-immunopositive neurons. The identified neurons revealed a distinct distribution pattern consisting of high cell density in the most rostral and caudal sections of the PPN nucleus, contrasting with lower densities in the medial segments. Our data also show a significant loss which affected PPN non-cholinergic cells, but not cholinergic ones in rats lesioned unilaterally in the Substantia Nigra pars compacta (SNpc) with a single injection of 6-hydroxydopamine (6-OHDA) compared to control animals. This result differs from previous studies which reported a substantial cholinergic cell loss in the PPN of post-mortem PD brains and in 6-OHDA-lesioned monkeys. Since a noted demise of dopaminergic neurons residing in the SN was confirmed in the 6-OHDA-lesioned rats, the current study suggests that a “dying-back” mechanism may underlie the cell death affecting non-cholinergic PPN neurons.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Neurology - Volume 248, October 2013, Pages 213-223
نویسندگان
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