Long-term running alleviates some behavioral and molecular abnormalities in Down syndrome mouse model Ts65Dn

► We studied the effect of running on the behavior and protein levels of Ts65Dn mice, a Down syndrome model. ► Cognitive functions improved only in mice that started running at weaning. ► Running initiated at both periods of life (post-weaning and adult) improved motor balance and memory of motor skills. ► Running affected the levels of some proteins, such as APP and SOD1, linked to Down syndrome.