Differential regulation of Nedd4 ubiquitin ligases and their adaptor protein Ndfip1 in a rat model of ischemic stroke

► HECT-type ubiquitin ligases are involved in neuron survival after ischemia. ► Ndfip1, Nedd4-2 and Itch are upregulated in surviving cells. ► Nedd4-1 is not upregulated during ischemia. ► Upregulation of Ndfip1 and Itch occurs separately in different cell types. ► Ndfip1 and Nedd4-2 show biochemical interaction in ischemia.