Na+-dependent transport of taurine is found only on the abluminal membrane of the blood-brain barrier

► Na+-dependent and facilitative transport of taurine were studied at the BBB. ► Taurine facilitative transport is present at the luminal and abluminal membranes. ► Na+-dependent transport of taurine is only present at the abluminal membrane. ► Na+-dependent transport of taurine is stimulated by membrane hyperpolarization. ► Na+-dependent transport of taurine is stimulated by hypotonicity.