کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
602844 | 879997 | 2006 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Denaturation of jack-bean urease by sodium n-dodecyl sulphate: A kinetic study below the critical micelle concentration
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Using a prepared computer program, the experimental data are properly fitted into a binomial exponential relation, considering a two-phase denaturation pathway including a kinetically stable folded intermediate formed at SDS concentration of 1.1 mM. Forward and backward rate constants are estimated as: k1 = 0.2141 ± 4.5 Ã 10â3, k2 = 5.173 Ã 10â3 ± 8.3 Ã 10â5, kâ1 = 0.09432 ± 3.6 Ã 10â4 and kâ2 = 2.079 Ã 10â3 ± 5.6 Ã 10â5 sâ1 for the proposed mechanism. The rate-limiting step as well as the reaction coordinates in the denaturation mechanism are established. The mechanism involves formation of a kinetically stable folded native like intermediate through the electrostatic interactions. The intermediate was found to be more stable even than the native form (by about 9 kJ molâ1) and still hexamer, because no loss of amplitude was observed. Electrophoresis experiments on the native and surfactant/urease complexes indicated a higher mobility for the kinetically folded native like intermediate.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 53, Issue 2, 1 December 2006, Pages 139-148
Journal: Colloids and Surfaces B: Biointerfaces - Volume 53, Issue 2, 1 December 2006, Pages 139-148
نویسندگان
K. Nazari, A. Mahmoudi, N. Esmaeili, L. Sadeghian, A.A. Moosavi-Movahedi, R. Khodafarin,