کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
604003 1454415 2017 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Thermal and rheological characteristics of mutant rice starches with widespread variation of amylose content and amylopectin structure
ترجمه فارسی عنوان
ویژگی های حرارتی و رئولوژیکی از جهش نشاسته برنج با تنوع گسترده از محتوای آمیلوز و ساختار آمیلوپکتین
کلمات کلیدی
رئولوژی؛ نشاسته؛ جهش برنج؛ ساختار آمیلوپکتین؛ اشعه ایکس؛ کالریمتری روبشی افتراقی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
چکیده انگلیسی


• SSIIIa and BEIIb deficiency did not affect the degree of crystallinity.
• BEIIb deficiency increased with gelatinization temperature.
• BEIIb deficiency accelerates retrogradation of the gel.
• Incomplete gelatinization enhances retrogradation.

Of starch biosynthesis-related enzymes, starch synthases (SSs) and starch branching enzymes (BEs) play important roles in the control of starch structure. Measurements of the particle size distribution, swelling power, differential scanning calorimetry (DSC), X-ray diffraction (XRD) and dynamic viscoelastic measurements were used to investigate the physicochemical properties of rice endosperm starch from SSIIIa, granule-bound starch synthase (GBSSI) and/or BEIIb deficient mutants including double mutants having widespread variation of amylose content and amylopectin structure. The predicted relative starch crystallinity (RSC) of A-type starches and B-type starches of these mutants, as computed based on amylopectin contents, were almost equal. These results suggest that SSIIIa and BEIIb deficiency does not affect the degree of crystallinity of amylopectin. A newly developed double mutant line (ss3a/be2b) with high amylose content (ca. 45%) and a lower proportion of amylopectin short chains showed a higher temperature of gelatinization. Moreover, retrogradation of the gel was extremely rapid. Additionally, be2b with extremely low proportion of amylopectin short chains and lower amylose contents (28%) showed higher gelatinization temperature and more rapid retrogradation than ss3a, with a lower proportion of amylopectin long chains with DP ≤ 33 and higher amylose content (30%). Granules of ss3a/be2b and be2b in gels after viscoelastic measurement were mostly maintained, indicating that most starch granules were not ruptured during heating. These results clarify that not only amylose contents but also the fine structure in amylopectin strongly affected rice gel viscoelastic properties.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Hydrocolloids - Volume 62, January 2017, Pages 83–93
نویسندگان
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