Comparison of characteristics of periodontal ligament cells obtained from outgrowth and enzyme-digested culture methods

PDL cells obtained by outgrowth and enzyme digestion showed different characteristics. The enzyme digestion method yielded cells with higher proliferation rate and mesenchymal stem cell-like properties, whereas cells with fibroblast-like properties were collected in the outgrowth method. PDL cell properties by different culture methods may provide information for inventing new therapeutic uses of PDL cells.