کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6055413 | 1198813 | 2012 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Clinical significance of molecular alterations in histologically negative surgical margins of head and neck cancer patients
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کلمات کلیدی
Gene expression - بیان ژنSurgical margins - حاشیه جراحیField cancerization - سرطان مزمنLocal neoplasm recurrence - عود عصب نواری محلیMatrix metalloproteinase 9 - ماتریکس متالوپروتئیناز 9Head and neck neoplasms - نئوپلاسم سر و گردنSecond primary neoplasms - نئوپلاسم های دوم دومParathyroid hormone-related protein - پروتئین مرتبط با هورمون پاراتیروئید
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
دندانپزشکی، جراحی دهان و پزشکی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Clinical significance of molecular alterations in histologically negative surgical margins of head and neck cancer patients Clinical significance of molecular alterations in histologically negative surgical margins of head and neck cancer patients](/preview/png/6055413.png)
چکیده انگلیسی
The development of locoregional recurrence is the main reason for treatment failure in head and neck squamous cell carcinomas (HNSCC) and the remaining of tumor cells in surgical margins is associated with recurrence. Surgical margins are considered negative based on histologic assessment of the pathological specimen. However, this method lacks sensitivity in identifying cells that already started malignant transformation but have not yet developed a pathologic phenotype. We investigated the usefulness of assessing the expression of PTHLH, EPCAM, MMP9, LGALS1 and MET for the detection of molecular alterations in histologically negative surgical margins and determine the correlation of these tumor-related alterations with clinical and prognostic parameters. Differential gene expression was determined by quantitative RT-PCR analyses in normal mucosa, HNSCC and negative margin samples. Thirty-eight percent of the histologically negative surgical margins examined were margin-positive for overexpression of at least one of the genes evaluated. Moreover, MMP9 and PTHLH overexpression in the surgical margins was associated with the development of second primary tumors (p = 0.002) and lower rates of local control (log rank test p = 0.022; HR = 4.186; p = 0.035), respectively. These findings demonstrate that the overexpression of tumor-related genes in histologically negative surgical margins is a frequent event. The use of qRT-PCR may be an useful tool in detecting actually negative HNSCC surgical margins and the overexpression of specific genes in these margins could be helpful in the identification of patients with a higher risk of developing second primary tumors and local recurrences, thus aiding the surgeon in the delineation of the HNSCC resection extent and helping in the planning of adjuvant therapy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Oral Oncology - Volume 48, Issue 3, March 2012, Pages 240-248
Journal: Oral Oncology - Volume 48, Issue 3, March 2012, Pages 240-248
نویسندگان
Ana Carolina de Carvalho, Luiz Paulo Kowalski, Antônio Hugo José Fróes Marques Campos, Fernando Augusto Soares, André Lopes Carvalho, André Luiz Vettore,