کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6087065 1589423 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Neuropathologic, phenotypic and functional analyses of Mucosal Associated Invariant T cells in Multiple Sclerosis
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Neuropathologic, phenotypic and functional analyses of Mucosal Associated Invariant T cells in Multiple Sclerosis
چکیده انگلیسی


- Similar frequency of MAIT cells in MS patients compared to HV except for PP patients.
- Unchanged phenotype and activation capacities in MS patients compared to HV.
- Low frequency of MAIT cells in MS lesions and a low transmigration ability.
- Expression of MR1, IL-18 and IL-23 was found in MS CNS lesions.

BackgroundThe involvement of Mucosal Associated Invariant T (MAIT) cells, which are anti-microbial semi-invariant T cells, remains elusive in Multiple Sclerosis (MS).ObjectiveDeciphering the potential involvement of MAIT cells in the MS inflammatory process.MethodsBy flow cytometry, blood MAIT cells from similar cohorts of MS patients and healthy volunteers (HV) were compared for frequency, phenotype, activation potential after in vitro TCR engagement by bacterial ligands and transmigration abilities through an in vitro model of blood-brain barrier. MS CNS samples were also studied by immunofluorescent staining and quantitative PCR.Results and conclusionBlood MAIT cells from relapsing-remitting MS patients and HV presented similar frequency, ex vivo effector phenotype and activation abilities. MAIT cells represented 0.5% of the total infiltrating T cells on 39 MS CNS lesions. This is low as compared to blood frequency (p < 0.001), but consistent with their low transmigration rate. Finally, transcriptional over-expression of MR1 - which presents cognate antigens to MAIT cells - and of the activating cytokines IL-18 and IL-23 was evidenced in MS lesions, suggesting that the CNS microenvironment is suited to activate the few infiltrating MAIT cells. Taken together, these data place MAIT cells from MS patients as minor components of the inflammatory pathological process.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinical Immunology - Volumes 166–167, May 2016, Pages 1-11
نویسندگان
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