کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6087289 | 1207354 | 2015 | 12 صفحه PDF | دانلود رایگان |
- MP4-induced EAE is characterized by B cell infiltration into the CNS.
- CNS infiltrating B cells show isotype switching associated with TH17 immunity.
- CNS infiltrating B cells show signs of somatic hypermutation.
- Epitope spreading of the CNS-reactive B cell response is a feature of the MP4 model.
In multiple sclerosis (MS) lymphoid follicle-like aggregates have been reported in the meninges of patients. Here we investigated the functional relevance of B cell infiltration into the central nervous system (CNS) in MP4-induced experimental autoimmune encephalomyelitis (EAE), a B cell-dependent mouse model of MS. In chronic EAE, B cell aggregates were characterized by the presence of CXCL13+ and germinal center CD10+ B cells. Germline transcripts were expressed in the CNS and particularly related to TH17-associated isotypes. We also observed B cells with restricted VH gene usage that differed from clones found in the spleen. Finally, we detected CNS-restricted spreading of the antigen-specific B cell response towards a myelin and a neuronal autoantigen. These data imply the development of autonomous B cell-mediated autoimmunity in the CNS in EAE - a concept that might also apply to MS itself.
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Journal: Clinical Immunology - Volume 158, Issue 1, May 2015, Pages 47-58