کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6087479 1207365 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Plasmocytoid dendritic cell deficit of early response to toll-like receptor 7 agonist stimulation in multiple sclerosis patients
ترجمه فارسی عنوان
غلظت سلول های دندریتیک پلاسموسیتوئید واکنش سریع به تحریک آگونیست گیرنده 7 مانند در بیماران مبتلا به مولتیپل اسکلروزیس
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
چکیده انگلیسی


- We analyzed plasmacytoid dendritic cells in relapsing-remitting multiple sclerosis.
- We stimulated cells via Toll-like receptor 7 (TLR7).
- We found a decrease of the early TLR7 agonist responses in RRMS.
- We found impaired Akt activation in pDCs in RRMS in early response to TLR7agonist.

Plasmacytoid dendritic cells (pDCs), an important immunoregulatory population, are characterized by vigorous secretion of type I interferons (IFNs) in response to toll-like receptor (TLR) 7 and 9 stimulation. We studied the function of pDCs in multiple sclerosis (MS) patients by analysis of TLR7 responses. We assessed a pDC secretion pattern of cytokines in the short term PBMC cultures stimulated with TLR7 agonist. pDCs sorted from PBMCs of both MS patients and controls were used to assess TLR7 expression profile. TLR7 induced signaling in pDCs has been analyzed with intracellular flow cytometry. We have identified a clinically correlated significant decrease of the TLR7-induced IFN-alfa (IFNa) secretion by pDCs from MS patients. This deficit has been accompanied by insufficient intracellular phosphorylation of protein kinase Akt and a decrease of the TLR7 gene expression in MS pDCs. Our results demonstrated a selective pDC deficit in MS supporting a relationship between pDCs and mechanisms of MS.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinical Immunology - Volume 153, Issue 1, July 2014, Pages 211-219
نویسندگان
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