کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6087677 1207376 2013 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Profiling celiac disease antibody repertoire
ترجمه فارسی عنوان
پروتئین ضد التهابی ضد سلیاک
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
چکیده انگلیسی


- We identify a new set of celiac disease antigens useful for antibody profiling
- This antibody response was not abolished on a gluten free diet
- These antigens were not shared with other autoimmune diseases.

The aim of this study was to dissect the autoantibody response in celiac disease (CD) that remains largely unknown, with the goal of identifying the disease-specific autoantigenic protein pattern or the so called epitome. Sera from CD patients were used to select immunoreactive antigens from a cDNA phage-display library. Candidate genes were identified, the corresponding proteins produced and their immunoreactivity validated with sera from CD patients and controls. Thirteen CD-specific antigens were identified and further validated by protein microarray. The specificity for 6 of these antigens was confirmed by ELISA. Furthermore we showed that this antibody response was not abolished on a gluten free diet and was not shared with other autoimmune diseases. These antigens appear to be CD specific and independent of gluten induction. The utility of this panel extends beyond its diagnostic value and it may drive the attention to new targets for unbiased screens in autoimmunity research.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinical Immunology - Volume 148, Issue 1, July 2013, Pages 99-109
نویسندگان
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