کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6087771 1207398 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Activated iNKT cells promote Vγ9Vδ2-T cell anti-tumor effector functions through the production of TNF-α
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Activated iNKT cells promote Vγ9Vδ2-T cell anti-tumor effector functions through the production of TNF-α
چکیده انگلیسی

Vγ9Vδ2-T cells constitute a proinflammatory lymphocyte subpopulation with established antitumor activity. Phosphoantigens activate Vγ9Vδ2-T cells in vivo and in vitro. We studied whether the antitumor activity of Vγ9Vδ2-T cells can be potentiated by invariant NKT cells (iNKT), an important immunoregulatory T cell subset. When activated by the glycolipid α-galactosylceramide (α-GalCer), iNKT produce large amounts of cytokines involved in antitumor immune responses. Monocyte-derived dendritic cells were loaded with both phosphoantigens (using aminobisphosphonates) and α-GalCer during maturation and subsequently co-cultured with Vγ9Vδ2-T and iNKT cells. Aminobisphosphonates dose-dependently enhanced Vγ9Vδ2-T cell activation, and this was potentiated by α-GalCer-induced iNKT co-activation. iNKT co-activation also enhanced the IFN-γ production and cytolytic potential of Vγ9Vδ2-T cells against tumor cells. Using transwell experiments and neutralizing antibodies cross-talk between iNKT and Vγ9Vδ2-T cells was found to be mediated by TNF-α. Our data provide a rationale for combining both activating ligands to improve Vγ9Vδ2-T cell based approaches in cancer-immunotherapy.

► Vγ9Vδ2-T cells are proinflammatory lymphocytes with established antitumor activity ► Immunoregulatory iNKT cells initiate and propagate antitumor immune responses ► Activated iNKT enhance Vγ9Vδ2-T activation, cytokine production and cytotoxicity ► Stimulation of Vγ9Vδ2-T by iNKT is cell-contact independent and mediated by TNF-α ► There is rationale for studying iNKT co-activation in Vγ9Vδ2-T based immunotherapy

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinical Immunology - Volume 142, Issue 2, February 2012, Pages 194-200
نویسندگان
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