کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6117196 1217170 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantification of V(D)J recombination by real-time quantitative PCR
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Quantification of V(D)J recombination by real-time quantitative PCR
چکیده انگلیسی
B and T lymphocytes have the unique capacity to somatically rearrange their antigen receptor loci through V(D)J recombination. D-JH and VH-DJH recombination events are usually visualized by semi-quantitative PCR followed by detection of end products, which is time consuming and requires the use of hazardous elements. Additionally, it necessitates relatively large amounts of genomic DNA which could be limiting when the cell populations of interest are rare. Here, we describe a real-time quantitative PCR assay for a fast quantification of V(D)J recombination events at the IgH locus.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunology Letters - Volume 162, Issue 1, Part A, November 2014, Pages 119-123
نویسندگان
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