CCR4 dependent migration of Foxp3+ Treg cells to skin grafts and draining lymph nodes is implicated in enhanced graft survival in CD200tg recipients

► Overexpression of CD200transgene increases allogeneic skin graft survival. ► Both graft tissue and draining lymph nodes show increased infiltration of Foxp3+ Treg. ► Gene expression profiling confirms increased expression of Treg-associated genes, as well as increased expression of genes encoding CCR4 and ligands, CCL17/22. ► Suppression of CCR4 expression with lentivirus encoding shRNA blocks Foxp3 Treg infiltration and increased skin graft survival.