کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6117442 1217205 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Antibody-dependent cell-mediated cytotoxicity is induced by a single-chain Fv-protein III fusion in the presence of a rabbit anti-protein III polyclonal antibody
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Antibody-dependent cell-mediated cytotoxicity is induced by a single-chain Fv-protein III fusion in the presence of a rabbit anti-protein III polyclonal antibody
چکیده انگلیسی
The use of phage-displayed antibody libraries has enabled the isolation of several thousand cancer-specific monoclonal antibodies. To further select for clones among these antibodies which have therapeutic potential for cancer, several types of in vitro anti-tumor assay, such as an antibody-dependent cell-mediated cytotoxicity (ADCC) assay, are required. The cytotoxic activities of effector cells are triggered by the binding of the Fc portion of IgG to its receptor, necessitating the conversion of a candidate clone with a single-chain variable fragment (scFv) form into a human IgG form. In the laboratory however, this conversion process is expensive and involves laborious steps such as the cloning of mammalian cells that contain an IgG expression vector, the subsequent production of protein, and affinity purification. In our current study, we show that an original fusion of scFv and protein III, a coat protein of the M13 bacteriophage, can induce ADCC activity towards its target cells in the presence of a rabbit anti-protein III polyclonal antibody. Our modified assay method thus enables the more rapid selection of potentially therapeutic clones from phage-displayed antibody libraries.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunology Letters - Volume 136, Issue 1, 30 April 2011, Pages 44-48
نویسندگان
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