Characterization of CD3+CD4−CD8− (double negative) T cells reconstitution in patients following hematopoietic stem-cell transplantation

► Dynamic CD3+CD4−CD8− (double negative) T cells reconstitution post-transplant. ► Successful expansion of DN Tregs at 60 days after allo-HCST avoid severe manifestations of aGVHD. ► DN Tregs have potential protection effect against aGVHD.