Conventional parasitology and DNA-based diagnostic methods for onchocerciasis elimination programmes

In this study, a qPCR assay was designed to test for Onchocerca volvulus DNA in dried skin snips after MF emerged. Results of this assay were compared to traditional diagnostic methods: microscopic detection of MF in skin snips and nodule palpation. The combination of molecular methods and traditional parasitological methods increases the prevalence estimation significantly.