Biological behavior of different Trypanosoma cruzi isolates circulating in an endemic area for Chagas disease in the Gran Chaco region of Argentina

► The biological behavior of Trypanosoma cruzi strains is still obscure. ► Four T. cruzi DTUs were found in the study area in Argentina. ► A vector transmission model was developed to maintain T. cruzi isolates. ► Different parasite load, serological response and tissue damage among isolates was detected. ► Results let us formulate hypotheses of clinical and epidemiological importance.