The sylvatic transmission cycle of Trypanosoma cruzi in a rural area in the humid Chaco of Argentina

► We identified the main sylvatic hosts of Trypanosoma cruzi in northeastern Argentina. ► Didelphis albiventris opossums and Dasypus novemcinctus armadillos were infected. ► Opossums were infected with T. cruzi I and armadillos with T. cruzi III. ► Panstrongylus geniculatus and Triatoma sordida were found in sylvatic habitats. ► Two independent sylvatic transmission cycles of T. cruzi occur in the humid Chaco.