Evaluation of real-time PCR for Strongyloides stercoralis and hookworm as diagnostic tool in asymptomatic schoolchildren in Cambodia

► We evaluate real-time PCR for soil-transmitted helminths in a cohort of asymptomatic children in Cambodia. ► We compare real-time PCR with microscopy and culture. ► Strongyloides spp. real-time PCR has good overall sensitivity and specificity. ► Multiple infections are identifiable by real-time PCR.