A simple immune complex dissociation ELISA for leishmaniasis: Standardization of the assay in experimental models and preliminary results in canine and human samples

► Leishmania serology is affected by the presence of immune complexes. ► Immune complexes are present in early stage of Leishmania chagasi experimental infection. ► Dissociation of immune complexes allows 2-5% increase in leishmaniasis IgG ELISA.