Trypanosoma cruzi I-III in southern Brazil causing individual and mixed infections in humans, sylvatic reservoirs and triatomines

► Genetic variability of T. cruzi in humans, and DTUs TcI, TcII and TcIII were found. ► For the first time, was observed DTU III in human in southern Brazil. ► Mixed infections (TcI and TcII) are found only in triatomines in southern Brazil.