Efficacy of parasitological methods for the diagnosis of Strongyloides stercoralis and hookworm in faecal specimens

► The occurrence of Strongyloides stercoralis and hookworm in faecal samples was investigated by 3 different methods. ► Agar plate culture sensitivity was assessed. ► Strongyloidiasis diagnosis was performed based on furrow patterns left by larvae on agar plate culture. ► The influence of stool refrigeration on Strongyloides larvae recovery was evaluated.