Use of recombinant proteins MPB70 or MPB83 as capture antigens in ELISAs to confirm bovine tuberculosis infections in Brazil

► Two indirect IgG-ELISAs using MPB70 or MPB83 as capture antigen were used for diagnosing tuberculosis in a naturally infected herd. ► Samples were collected in three different times in order to investigate the usage of ELISA as a confirmatory test. ► Both presented similar results (kappa statistic = 0.92) among the three times of the study. ► ELISAs were proved to be a valuable complementary tool to intradermal testing. ► However, ELISAs were not considered as reliable indicators of the early phases of this infection.