Cloning, expression, purification and kinetics of trehalose-6-phosphate phosphatase of filarial parasite Brugia malayi

► Bm-TPP was cloned, expressed and found to possess robust phosphatase activity. ► It shows specificity towards trehalose-6-phosphate and belongs to HAD super family. ► The enzyme shows absolute requirement for Mg2+ as a metal ion for activity.