کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6129363 1222155 2015 16 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular identification of Mucorales in human tissues: contribution of PCR electrospray-ionization mass spectrometry
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
پیش نمایش صفحه اول مقاله
Molecular identification of Mucorales in human tissues: contribution of PCR electrospray-ionization mass spectrometry
چکیده انگلیسی
Molecular methods are crucial for mucormycosis diagnosis because cultures are frequently negative, even if microscopy suggests the presence of hyphae in tissues. We assessed PCR/electrospray-ionization mass spectrometry (PCR/ESI-MS) for Mucorales identification in 19 unfixed tissue samples from 13 patients with proven or probable mucormycosis and compared the results with culture, quantitative real-time PCR, 16S-23S rRNA gene internal transcribed spacer region (ITS PCR) and 18S PCR sequencing. Concordance with culture identification to both genus and species levels was higher for PCR/ESI-MS than for the other techniques. Thus, PCR/ESI-MS is suitable for Mucorales identification, within 6 hours, for tissue samples for which microscopy results suggest the presence of hyphae.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinical Microbiology and Infection - Volume 21, Issue 6, June 2015, Pages 594.e1-594.e5
نویسندگان
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