کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6129464 1222158 2015 24 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Microbial diagnosis of bloodstream infection: towards molecular diagnosis directly from blood
ترجمه فارسی عنوان
تشخیص میکروبی عفونت خون: به طور مستقیم به تشخیص مولکولی از خون
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
چکیده انگلیسی
When a bloodstream infection (BSI) is suspected, most of the laboratory results-biochemical and haematologic-are available within the first hours after hospital admission of the patient. This is not the case for diagnostic microbiology, which generally takes a longer time because blood culture, which is to date the reference standard for the documentation of the BSI microbial agents, relies on bacterial or fungal growth. The microbial diagnosis of BSI directly from blood has been proposed to speed the determination of the etiological agent but was limited by the very low number of circulating microbes during these paucibacterial infections. Thanks to recent advances in molecular biology, including the improvement of nucleic acid extraction and amplification, several PCR-based methods for the diagnosis of BSI directly from whole blood have emerged. In the present review, we discuss the advantages and limitations of these new molecular approaches, which at best complement the culture-based diagnosis of BSI.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinical Microbiology and Infection - Volume 21, Issue 4, April 2015, Pages 323-331
نویسندگان
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