کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6133255 | 1593457 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A rapid immunochromatographic test to detect the lily mottle virus
ترجمه فارسی عنوان
یک آزمایش ایمونو کروماتوگرافی سریع برای تشخیص ویروس مگس لیلی
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
چکیده انگلیسی
We developed a rapid immunochromatographic strip (ICS) test for lily mottle virus (LMoV). The test is based on a double-antibody sandwich format and employs two distinct anti-LMoV polyclonal antibodies (IgG3 and IgG4). The first antibody, IgG3 was conjugated with colloidal gold, and the second antibody, IgG4 was used as the capture antibody at the test line. The performance of the ICS test was evaluated and the results obtained were compared with a quadruplex RT-PCR assay. When serial dilutions of purified LMoV were tested, the LMoV detection limit of the ICS test was 8.0 Ã 10â9 mg/mL, which was in complete agreement with the results of quadruplex RT-PCR. Compared with quadruplex RT-PCR, the specificity and sensitivity of ICS were 98.7 and 100%, respectively. There was therefore significant agreement between the results obtained from the two tests (κ = 0.982). The ICS test therefore appears to be broadly applicable, and will be especially useful in the field, as well as in areas without laboratory facilities, to support efforts to detect and control LMoV.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 220, August 2015, Pages 43-48
Journal: Journal of Virological Methods - Volume 220, August 2015, Pages 43-48
نویسندگان
Yubao Zhang, Yajun Wang, Wanrong Yang, Zhongkui Xie, Ruoyu Wang, Hadley Randal Kutcher, Zhihong Guo,