کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6133287 1593458 2015 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Short communicationDevelopment of a stable Gaussia luciferase enterovirus 71 reporter virus
ترجمه فارسی عنوان
ارتباطات کوتاه توسعه یک ویروس خبرنگار گواسی لوسیفراز انتروویروس 71
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
چکیده انگلیسی


- A Gaussia luciferase EV71 reporter virus was constructed.
- The reporter virus was infectious and stable in cell culture.
- The reporter virus was suitable for antiviral test.

We report a stable Gaussia luciferase enterovirus 71 (Gluc-EV71) reporter virus to facilitate drug discovery. The Gluc-EV71 reporter virus was generated by engineering the Gaussia luciferase (Gluc) gene between the 5′ untranslated region and VP4 gene of the EV71 genome. We could recover Gluc-EV71 after transfection of Vero cells with the cDNA clone-derived RNA. The reporter virus efficiently infects and replicates in various cell types (Vero, human rhabdomyosarcoma, and HeLa cells), producing robust luciferase activity. The Gluc-EV71 virus replicates slower than the wild-type virus in cell culture. The reporter virus is stable in maintaining the Gluc gene after five rounds of continuous passaging in Vero cells. Using known EV71 inhibitors, we demonstrate that the reporter virus can be used for antiviral testing. However, the Gluc-EV71 infection assay cannot be adapted to a homogenous format for high throughput screen, mainly due to the secreted nature of the Gluc protein and the short half-life of the Gluc luminescence signal. The Gluc-EV71 and its infection assay could be useful for antiviral drug discovery as well as for studying EV71 replication and pathogenesis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 219, July 2015, Pages 62-66
نویسندگان
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