کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6134140 | 1223578 | 2013 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A rapid method for infectivity titration of Andes hantavirus using flow cytometry
ترجمه فارسی عنوان
یک روش سریع برای تفتیش عفونت زایی آندز هانتاویروس با استفاده از جریان سیاتومتری
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کلمات کلیدی
هانتاویروس، هسته پروتئین، تثبیت ویروس عفونی، تمرکز تست، جریان سیاتومتری،
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
چکیده انگلیسی
The focus assay is currently the most commonly used technique for hantavirus titer determination. This method requires an incubation time of between 5 and 11 days to allow the appearance of foci after several rounds of viral infection. The following work presents a rapid Andes virus (ANDV) titration assay, based on viral nucleocapsid protein (N) detection in infected cells by flow cytometry. To this end, an anti-N monoclonal antibody was used that was developed and characterized previously. ANDV N could be detected as early as 6Â h post-infection, while viral release was not observed until 24-48Â h post-infection. Given that ANDV detection was performed during its first round of infection, a time reduction for titer determination was possible and provided results in only two days. The viral titer was calculated from the percentage of N positive cells and agreed with focus assay titers. Furthermore, the assay was applied to quantify the inhibition of ANDV cell entry by patient sera and by preventing endosome acidification. This novel hantavirus titration assay is a highly quantitative and sensitive tool that facilitates infectivity titration of virus stocks, rapid screening for antiviral drugs, and may be further used to detect and quantify infectious virus in human samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 193, Issue 2, November 2013, Pages 291-294
Journal: Journal of Virological Methods - Volume 193, Issue 2, November 2013, Pages 291-294
نویسندگان
Gonzalo P. Barriga, Constanza MartÃnez-Valdebenito, Héctor Galeno, Marcela Ferrés, Pierre-Yves Lozach, Nicole D. Tischler,