Recombinant glycoprotein G analog for determination of specific immunoglobulins to herpes simplex virus type 2 by ELISA

The purified recombinant HSV-2 antigen was used to design a diagnostic ELISA kit, which was evaluated with referent controls and standard panels of sera containing and/or not containing anti-HSV-2 IgG. Comparative evaluation of this kit and the commercially available “HSV-Type 2 IgG-ELISA” (NovaTec, Dietzenbach, Germany) kit was performed. There was no significant difference (P > 0.05). It allows to use developed ELISA kit for clinical diagnosis of HSV-2 infection.