Inactivation of infectious virus and serological detection of virus antigen in Rift Valley fever virus-exposed mosquitoes fixed with paraformaldehyde

► Infectious RVFV in mosquitoes was inactivated by paraformaldehyde. ► Paraformaldehyde fixation inhibited immunological detection of RVFV in mosquitoes. ► Incubation of mosquito sections with trypsin successfully unmasked viral antigens.