A time-resolved immunoassay to measure serum antibodies to the rotavirus VP6 capsid protein

► Two recombinant VP6 capsid proteins from an Indian birth cohort were expressed. ► The proteins were used to establish a time-resolved fluorescence DELFIA. ► DELFIA was compared to a gold standard ELISA using cohort serum samples. ► A significant association was observed between the two assays (p < 0.05). ► This demonstrates recombinant proteins can be used to study RV antibody responses.