A strand specific real-time RT-PCR method for the targeted detection of the three species (vRNA, cRNA and mRNA) of infectious salmon anaemia virus (ISAV) replicative RNA

► ISAV RNA strand specific real-time RT-PCR development. ► Distinguishes the 3 species of ISAV replicative RNA - vRNA, cRNA and mRNA. ► Tested on artificial T7-promoter produced transcripts. ► Applied to infected cells in culture with RNA populations varied by temperature or chemical inhibitor.