Development and evaluation of a real-time reverse transcription-loop-mediated isothermal amplification assay for rapid serotyping of foot-and-mouth disease virus

► RT-LAMP for rapid detection and serotyping of FMD virus was developed. ► We reported for the first time that serotyping of FMDV O and A by RT-LAMP. ► RT-LAMP assay could detect and serotype FMDV samples stored in FTA® cards.