Routine use of duplex real-time PCR assays including a commercial internal control for molecular diagnosis of opportunistic DNA virus infections

► Improving the validity of laboratory-developed real-time PCRs for the detection/quantitation of DNA viruses. ► Use of the commercial Simplexa™ extraction and amplification control (SEAC) set. ► The SEAC showed high reproducibility and high stability. ► Procedure for validation of PCR results in quality assurance programs.