Detection of neutralizing antibodies against bluetongue virus serotype 8 by an optimized plasma neutralization test

► Plasma neutralization test optimized for detection of anti-blue tongue virus antibody. ► Low neutralizing antibody production after serotype 8 infection. ► Reduced TCID50 retains test sensitivity for samples with low antibody titer. ► Virus stock production and storage impact test performance. ► Test development based on plasma, not serum.