Development of an indirect ELISA for serological detection of reticuloendotheliosis virus using the gp90 protein expressed in Pichia pastoris

► The gp90 protein of reticuloendotheliosis virus (REV) was expressed in P. pastoris. ► The expressed gp90 protein was secreted into the medium with good antigenicity. ► An novel indirect ELISA was established using the gp90 protein as coating antigen. ► The gp90-ELISA displays good sensitivity, specificity and reproducibility. ► The gp90-ELISA shows advantages over virus neutralization and the whole virus based ELISA.