Comparative procedures for sample processing and quantitative PCR detection of grapevine viruses

► Comparison of grapevine tissue preparation methods. ► Comparison of different RNA extraction methods for RT-qPCR. ► Comparison of different RT-qPCR methods used for detection of viruses in grapevine. ► Comparison of different reagents used in RT-qPCR for the detection of grapevine viruses.