Rapid and sensitive detection of Laem-Singh virus by reverse transcription loop-mediated isothermal amplification combined with a lateral flow dipstick

► We have developed a rapid RT-LAMP-LFD test for detecting LSNV infections in shrimp. ► The RT-LAMP also provided ∼1000-fold greater LSNV RNA detection sensitivity compared to the RT-PCR, and it did not cross-detect other RNA viruses commonly found in shrimp farmed in Southeast Asia. ► The use of a LFD to detect LSNV DNA amplified by the RT-LAMP afforded additional simplicity, reduced the time needed to generate a result and negated the need for agarose electrophoresis and DNA detection equipment.