Development of a recombinant truncated nucleocapsid protein based immunoassay for detection of antibodies against human coronavirus OC43

► The diagnostic sensitivity and specificity of the HCoV-OC43 truncated N immunoassay were 90.9% (10/11) and 82.9% (39/47), respectively. ► The assay demonstrated 100% diagnosis agreement with the previous testing methods. ► This assay offers may be a useful tool in coronavirus seroepidemiological studies.