A specific and sensitive antigen capture assay for NS1 protein quantitation in Japanese encephalitis virus infection

► Production of high amount of soluble hexameric form of NS1 glycoprotein of Japanese encephalitis virus. ► Characterization of a highly specific monoclonal antibody against a conformational epitope of NS1 protein of Japanese encephalitis virus genotypes I to IV. ► New diagnosis ELISA test highly sensitive and specific for Japanese encephalitis detection in biological samples.