A hybrid-capture assay to detect HPV mRNA ratios in cervical specimens

► This HPV mRNA hybrid-capture assay has use in HPV transcription studies. ► The analytical performance for in vitro RNA and cultured cells was satisfactory. ► Sample size and no longitudinal arm limited the clinical assessment of assay. ► Cellular heterogeneity affects the composite mRNA ratio of samples. ► Cell collection by passive adsorption was useful for cervical samples.